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In this study, researchers analyzed the effects of inserting a P element into the CG3011 gene on sleep duration in Drosophila melanogaster. They found that sleep duration was decreased when a P element was inserted into the CG3011 gene. Further analysis revealed that CG3011 encodes the serine hydroxymethyltransferase (SHMT) enzyme, which is involved in the synthesis of L-Ser25,26. The researchers generated mutations in the shmt gene using CRISPR-Cas9 and found that deleting all three isoforms of SHMT caused lethality, while introducing a STOP codon in the first coding exon of isoform A resulted in viable mutants. The mRNA level of isoform A SHMT was significantly decreased in the shmt-es mutants compared to wild type flies. The study used backcrossing to introduce the mutations into an isogenic Canton-S (CS) line and conducted further analysis on these mutants.
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Связанные резюме

The article discusses the importance of sleep in animals and the lack of understanding about its homeostatic regulation. Drosophila has been used as a model for genetic studies o…
In this study, researchers analyzed the effects of inserting a P element into the CG3011 gene on sleep duration in Drosophila melanogaster. They found that sleep duration was dec…
In a study on fruit fly behavior, researchers used video recording and analysis to measure sleep in both wild-type and mutant flies. Under a 12-hour light/12-hour dark cycle (LD …
SR is responsible for D-Ser production in vivo and a specific gene called CG8129 encodes this protein in Drosophila. To investigate the role of D-Ser, researchers generated flies…
Researchers studied the sleep patterns of fruit flies with a mutation in the "SRKO" gene and compared them to normal flies (wt). They found that the SRKO flies had a higher arous…
The text discusses the role of an enzyme called SR in the synthesis of two forms of serine, L-serine and D-serine, which are involved in sleep regulation. The study found that SR…
The article discusses recent research on the role of the gut-brain axis in sleep regulation and its implications for neurological disorders. The authors propose that the gut micr…
In this article, the authors discuss the complex relationships between chromatin loops, gene positioning, and gene expression. They explain that chromatin is organized into loops,…
Researchers have discovered that the amino acid D-serine plays a role in regulating sleep patterns. While D-serine was first detected in animals over two decades ago, little is k…